Use of Aqueous Two-Phase System for Partial Purification and Characterization of α-Amylase from Burnatia enneandra Micheli (2019)
Abstract: Alpha-amylase from Burnatia enneandra Micheli, a tuber plant from a rural area of Cameroon, extracted under optimal conditions was purified using aqueous two-phase extraction system (ATPS). The separation behaviour was further investigated. Polyethylene glycol (PEG, % w/w), ammonium sulphate (AS, % w/w) and pH were optimized for the purification of B. enneandra alpha-amylase. Optimum conditions for purification factor were PEG, 13.1 %, AS, 12 % and pH 4. Highest enzyme recovery was achieved at PEG, 13.0 %, AS, 12 % and pH 5.1. Further, enzyme characterization surveys acknowledged that alpha-amylase formed was just an acidic enzyme. The pH and temperature at which the enzyme was well expressed were 5.5 and 70 °C respectively. Another characteristic of the enzyme was determined like the Michaelis-Menten constant (Km) and Vmax which value obtained was respectively 89.32 μg/mL and 2.99 μg/mL/min.
